diff --git a/.changeset/fix-popper-anchor-positioning.md b/.changeset/fix-popper-anchor-positioning.md
new file mode 100644
index 000000000..b8d79e56f
--- /dev/null
+++ b/.changeset/fix-popper-anchor-positioning.md
@@ -0,0 +1,5 @@
+---
+'@radix-ui/react-popper': patch
+---
+
+Fixed `Popper.Anchor` positioning when `PopoverAnchor` and `PopoverTrigger` coexist as siblings or with CSS absolute positioning
diff --git a/apps/storybook/stories/popover.stories.tsx b/apps/storybook/stories/popover.stories.tsx
index 7aaca1343..a6d2dd7a8 100644
--- a/apps/storybook/stories/popover.stories.tsx
+++ b/apps/storybook/stories/popover.stories.tsx
@@ -267,6 +267,38 @@ export const CustomAnchor = () => (
   </Popover.Root>
 );
 
+export const CustomAnchorSibling = () => (
+  <Popover.Root>
+    <Popover.Anchor
+      style={{
+        display: 'flex',
+        justifyContent: 'space-between',
+        alignItems: 'center',
+        width: 250,
+        padding: 20,
+        margin: 100,
+        backgroundColor: '#eee',
+      }}
+    >
+      Item
+    </Popover.Anchor>
+    <Popover.Trigger className={styles.trigger} style={{ margin: '0 100px' }}>
+      open
+    </Popover.Trigger>
+    <Popover.Portal>
+      <Popover.Content
+        className={styles.content}
+        side="right"
+        sideOffset={1}
+        align="start"
+        style={{ borderRadius: 0, width: 200, height: 100 }}
+      >
+        <Popover.Close>close</Popover.Close>
+      </Popover.Content>
+    </Popover.Portal>
+  </Popover.Root>
+);
+
 export const WithSlottedTrigger = () => {
   return (
     <Popover.Root>
diff --git a/packages/react/popper/src/popper.tsx b/packages/react/popper/src/popper.tsx
index efb8cc26b..1695d065b 100644
--- a/packages/react/popper/src/popper.tsx
+++ b/packages/react/popper/src/popper.tsx
@@ -77,15 +77,13 @@ const PopperAnchor = React.forwardRef<PopperAnchorElement, PopperAnchorProps>(
     const ref = React.useRef<PopperAnchorElement>(null);
     const composedRefs = useComposedRefs(forwardedRef, ref);
 
-    const anchorRef = React.useRef<Measurable | null>(null);
     React.useEffect(() => {
-      const previousAnchor = anchorRef.current;
-      anchorRef.current = virtualRef?.current || ref.current;
-      if (previousAnchor !== anchorRef.current) {
-        // Consumer can anchor the popper to something that isn't
-        // a DOM node e.g. pointer position, so we override the
-        // `anchorRef` with their virtual ref in this case.
-        context.onAnchorChange(anchorRef.current);
+      // Consumer can anchor the popper to something that isn't
+      // a DOM node e.g. pointer position, so we override the
+      // `anchorRef` with their virtual ref in this case.
+      const desiredAnchor = virtualRef?.current || ref.current;
+      if (context.anchor !== desiredAnchor) {
+        context.onAnchorChange(desiredAnchor);
       }
     });