diff --git a/app/helper/directory.py b/app/helper/directory.py
index ee95ad40ee..c40695a1f3 100644
--- a/app/helper/directory.py
+++ b/app/helper/directory.py
@@ -74,7 +74,8 @@ def get_download_dir_by_save_path(
                 return dir_info
             if dir_info.media_type == media_type and not dir_info.media_category:
                 return dir_info
-            if dir_info.media_type == media_type and dir_info.media_category == media.category:
+            if dir_info.media_type == media_type and _match_media_category(
+                    dir_info.media_category, media.category):
                 return dir_info
         return None
 
@@ -136,8 +137,8 @@ def get_dir(self, media: Optional[MediaInfo], include_unsorted: Optional[bool] =
             if d.media_type == media_type and not d.media_category:
                 matched_dirs.append(d)
                 continue
-            # 目录类型相等，目录类别相等，符合条件
-            if d.media_type == media_type and d.media_category == media.category:
+            # 目录类型相等，目录类别命中配置，符合条件
+            if d.media_type == media_type and _match_media_category(d.media_category, media.category):
                 matched_dirs.append(d)
                 continue
         if matched_dirs:
@@ -210,6 +211,20 @@ def get_media_root_path(rename_format: str, rename_path: Path) -> Optional[Path]
         return media_root
 
 
+def _match_media_category(configured_category: Optional[str], media_category: Optional[str]) -> bool:
+    """
+    判断媒体类别是否命中目录配置，支持逗号分隔的多个类别。
+    """
+    if not configured_category or not media_category:
+        return False
+    categories = {
+        category.strip()
+        for category in re.split(r"[,，]", configured_category)
+        if category.strip()
+    }
+    return media_category.strip() in categories
+
+
 def _split_file_uri(value: str) -> Tuple[str, str]:
     """
     拆分 FileURI 字符串，保留原始路径用于安全校验。
diff --git a/tests/test_directory.py b/tests/test_directory.py
new file mode 100644
index 0000000000..37880b16f8
--- /dev/null
+++ b/tests/test_directory.py
@@ -0,0 +1,72 @@
+from pathlib import Path
+from types import SimpleNamespace
+
+import pytest
+
+from app.helper.directory import DirectoryHelper
+from app.schemas import TransferDirectoryConf
+
+
+def _media(category: str):
+    """构造目录匹配测试所需的媒体信息。"""
+    return SimpleNamespace(
+        type=SimpleNamespace(value="电视剧"),
+        category=category,
+    )
+
+
+def _directory(media_category: str) -> TransferDirectoryConf:
+    """构造启用自动整理的动漫目录配置。"""
+    return TransferDirectoryConf(
+        name="动漫",
+        priority=1,
+        storage="local",
+        download_path="/media/anime",
+        media_type="电视剧",
+        media_category=media_category,
+        monitor_type="monitor",
+        library_path="/media/link/anime",
+        library_storage="local",
+    )
+
+
+@pytest.mark.parametrize("configured_category", ["日番", "日番,日韩剧", "日番，日韩剧", " 日番 , 日韩剧 "])
+def test_get_dir_matches_media_category_list(monkeypatch, configured_category):
+    """自动整理应匹配目录配置中逗号分隔的任一媒体类别。"""
+    directory = _directory(configured_category)
+    monkeypatch.setattr(DirectoryHelper, "get_dirs", staticmethod(lambda: [directory]))
+
+    matched = DirectoryHelper().get_dir(
+        media=_media("日番"),
+        storage="local",
+        src_path=Path("/media/anime/demo.mkv"),
+    )
+
+    assert matched == directory
+
+
+def test_get_dir_rejects_unconfigured_media_category(monkeypatch):
+    """自动整理不应匹配目录配置中不存在的媒体类别。"""
+    directory = _directory("日番,日韩剧")
+    monkeypatch.setattr(DirectoryHelper, "get_dirs", staticmethod(lambda: [directory]))
+
+    matched = DirectoryHelper().get_dir(
+        media=_media("欧美剧"),
+        storage="local",
+        src_path=Path("/media/anime/demo.mkv"),
+    )
+
+    assert matched is None
+
+
+def test_get_download_dir_by_save_path_matches_media_category_list(monkeypatch):
+    """精确保存根路径应继承逗号分隔的媒体类别规则。"""
+    directory = _directory("日番,日韩剧")
+    monkeypatch.setattr(DirectoryHelper, "get_download_dirs", lambda _self: [directory])
+
+    matched = DirectoryHelper().get_download_dir_by_save_path(
+        media=_media("日韩剧"),
+        save_path="/media/anime",
+    )
+
+    assert matched == directory