probetools makeprobes -t target_space_FASTA.fa -b 100 -o demo_probes_dir/demo_probes

[ramnageena11]

I am trying to design probes for 15 sequences..

got the error regarding Vsearch.....

probetools makeprobes -t /home/gilbert/Documents/ncbi_dataset/data/GCF_904425475.1/cds_sortd_mg1655.fna -b 1 -o /home/gilbert/Documents/probes-output/mg1655-probes.fa -k 50 -i 50

ProbeTools MakeProbes v0.1.11
https://github.com/KevinKuchinski/ProbeTools

Extracting target names and seqs from /home/gilbert/Documents/ncbi_dataset/data/GCF_904425475.1/cds_sortd_mg1655.fna...
Total targets loaded: 15

******************** ROUND 1 ********************

Extracting low coverage areas...
Wrote 15 low coverage seqs from 15 targets.

Enumerating all 50-mers in /home/gilbert/Documents/probes-output/mg1655-probes.fa_low_cov_seqs.fa...
Loaded 15 target seqs in /home/gilbert/Documents/probes-output/mg1655-probes.fa_low_cov_seqs.fa...
Enumerated 15,009 k-mers from /home/gilbert/Documents/probes-output/mg1655-probes.fa_low_cov_seqs.fa.
Clustering k-mers at 50.0% identity...

ERROR: vsearch cluster_fast terminated with errors while clustering k-mers (Error code: 127).